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competent cell kit  (Zymo Research)


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    Structured Review

    Zymo Research competent cell kit
    Competent Cell Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mix+go+competent+e+coli+cells/Mix+%26+Go!+E%2Ecoli+Transformation+Kit+(Zymobroth+not+included)/pmc12857616-189-38-42
    Average 95 stars, based on 219 article reviews
    competent cell kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Silver Nanoparticles Templated by the M13 Phage Exhibit High Antibacterial Activity against Gram-Negative Pathogens and a Reduced Rate of Bacterial Resistance In Vitro.
    Article Snippet: Mutagenic oligonucleotides were designed using the Agilent QuikChange Primer design program.

    Mutagenesis:

    Article Title: Silver Nanoparticles Templated by the M13 Phage Exhibit High Antibacterial Activity against Gram-Negative Pathogens and a Reduced Rate of Bacterial Resistance In Vitro
    Article Snippet: Mutagenic oligonucleotides were designed using the Agilent QuikChange Primer design program. .. The Y21A mutant was constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA), according to the manufacturer’s instructions, and transformed into Mix & Go Competent E. coli Cells (Zymo Research, USA) using kanamycin selection. .. Mutagenic oligonucleotides for this mutation were designed in Benchling ( https://www.benchling.com/ ).

    Construct:

    Article Title: Silver Nanoparticles Templated by the M13 Phage Exhibit High Antibacterial Activity against Gram-Negative Pathogens and a Reduced Rate of Bacterial Resistance In Vitro
    Article Snippet: Mutagenic oligonucleotides were designed using the Agilent QuikChange Primer design program. .. The Y21A mutant was constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA), according to the manufacturer’s instructions, and transformed into Mix & Go Competent E. coli Cells (Zymo Research, USA) using kanamycin selection. .. Mutagenic oligonucleotides for this mutation were designed in Benchling ( https://www.benchling.com/ ).

    Transformation Assay:

    Article Title: Silver Nanoparticles Templated by the M13 Phage Exhibit High Antibacterial Activity against Gram-Negative Pathogens and a Reduced Rate of Bacterial Resistance In Vitro
    Article Snippet: Mutagenic oligonucleotides were designed using the Agilent QuikChange Primer design program. .. The Y21A mutant was constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA), according to the manufacturer’s instructions, and transformed into Mix & Go Competent E. coli Cells (Zymo Research, USA) using kanamycin selection. .. Mutagenic oligonucleotides for this mutation were designed in Benchling ( https://www.benchling.com/ ).

    Selection:

    Article Title: Silver Nanoparticles Templated by the M13 Phage Exhibit High Antibacterial Activity against Gram-Negative Pathogens and a Reduced Rate of Bacterial Resistance In Vitro
    Article Snippet: Mutagenic oligonucleotides were designed using the Agilent QuikChange Primer design program. .. The Y21A mutant was constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA), according to the manufacturer’s instructions, and transformed into Mix & Go Competent E. coli Cells (Zymo Research, USA) using kanamycin selection. .. Mutagenic oligonucleotides for this mutation were designed in Benchling ( https://www.benchling.com/ ).



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    Zymo Research e coli dh5 alpha competent cells
    ( A ) Frequency of aSD-like 8mers (set size = 882; free energy of binding to the consensus SD less than −8 kcal/mol) in endogenous B. subtilis CDSs (n = 4,218) compared to frequency of an equally sized set of control 8mers randomly sampled. p- value was computed from a two-sided Mann-Whitney U test. ( B ) Positional frequency over 1000 nt, 3-nt bins, of the frequency of aSD-like 8mers in endogenous B. subtilis CDSs compared to the control from A . Inset: 100-nt window. ( C ) Same as A for endogenous <t>E.</t> <t>coli</t> CDSs (n = 4,146). ( D-F ) Frequency of aSD-like 8mers in B. subtilis CDSs compared to their frequency in nucleotide shuffled CDSs ( D ), triplet shuffled CDSs ( E ), and codon shuffled CDSs ( F ). p -values were computed from paired two-sided Student’s t -tests. Mean frequencies from 20 independent shuffling instances are reported. ( G-H ) Same as F for E. coli CDSs ( G ) and leaderless CDS of C. crescentus (n = 374) ( H ).
    E Coli Dh5 Alpha Competent Cells, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mix+go+competent+e+coli+cells/Mix+%26+Go!+Competent+Cells+-+DH5+Alpha/bio_rxiv__2025__11__27__690947-161-0-6
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    Image Search Results


    ( A ) Frequency of aSD-like 8mers (set size = 882; free energy of binding to the consensus SD less than −8 kcal/mol) in endogenous B. subtilis CDSs (n = 4,218) compared to frequency of an equally sized set of control 8mers randomly sampled. p- value was computed from a two-sided Mann-Whitney U test. ( B ) Positional frequency over 1000 nt, 3-nt bins, of the frequency of aSD-like 8mers in endogenous B. subtilis CDSs compared to the control from A . Inset: 100-nt window. ( C ) Same as A for endogenous E. coli CDSs (n = 4,146). ( D-F ) Frequency of aSD-like 8mers in B. subtilis CDSs compared to their frequency in nucleotide shuffled CDSs ( D ), triplet shuffled CDSs ( E ), and codon shuffled CDSs ( F ). p -values were computed from paired two-sided Student’s t -tests. Mean frequencies from 20 independent shuffling instances are reported. ( G-H ) Same as F for E. coli CDSs ( G ) and leaderless CDS of C. crescentus (n = 374) ( H ).

    Journal: bioRxiv

    Article Title: Long-range mRNA folding shapes expression and sequence of bacterial genes

    doi: 10.1101/2025.11.27.690947

    Figure Lengend Snippet: ( A ) Frequency of aSD-like 8mers (set size = 882; free energy of binding to the consensus SD less than −8 kcal/mol) in endogenous B. subtilis CDSs (n = 4,218) compared to frequency of an equally sized set of control 8mers randomly sampled. p- value was computed from a two-sided Mann-Whitney U test. ( B ) Positional frequency over 1000 nt, 3-nt bins, of the frequency of aSD-like 8mers in endogenous B. subtilis CDSs compared to the control from A . Inset: 100-nt window. ( C ) Same as A for endogenous E. coli CDSs (n = 4,146). ( D-F ) Frequency of aSD-like 8mers in B. subtilis CDSs compared to their frequency in nucleotide shuffled CDSs ( D ), triplet shuffled CDSs ( E ), and codon shuffled CDSs ( F ). p -values were computed from paired two-sided Student’s t -tests. Mean frequencies from 20 independent shuffling instances are reported. ( G-H ) Same as F for E. coli CDSs ( G ) and leaderless CDS of C. crescentus (n = 374) ( H ).

    Article Snippet: E. coli DH5 Alpha Competent Cells (Zymo) and isolated via QIAprep Spin Miniprep Kit (Qiagen).

    Techniques: Binding Assay, Control, MANN-WHITNEY